You have no items in your shopping cart.
Featured
Description
Research Area
Metabolism Research
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Reactivity | Human, Rat |
| Predicted Reactivity | Gallus, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-JMJD1A antibody (orb1239525) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human JMJD1A. The immunogen is located within amino acids 50-100 of JMJD1A. |
| Target | KDM3A |
| Molecular Weight | Predicted: 147kDObserved: 147kD |
| Purification | JMJD1A Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | JMJD1A Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−JMJD1A Antibody: TSGA, JMJD1, JHDM2A, JHMD2A, JMJD1A, KIAA0742, TSGA, Lysine-specific demethylase 3A, JmjC domain-containing histone demethylation protein 2A
Similar Products
−- Item 1 of 1
Human Lysine Specific Demethylase 3A (KDM3A) ELISA Kit [orb1736545]
Human
0.16-10 ng/mL
0.054 ng/mL
96 T, 48 T 
- Item 1 of 3
KDM3A Rabbit Polyclonal Antibody [orb395225]
ELISA, IF, IHC, IP, WB
Human, Mouse
Rabbit
Polyclonal
Unconjugated
50 μg, 100 μg - Item 1 of 2
- Item 1 of 3
JMJD1A Rabbit Polyclonal Antibody [orb574439]
IHC, WB
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish
Human
Rabbit
Polyclonal
Unconjugated
100 μl
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: JMJD1A, orb1239525 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: JMJD1A, orb1239525 (2 µg/mL), JMJD1A (2 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human HepG2 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: JMJD1A, orb1239525 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of JMJD1A in Rat Liver Tissue. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-JMJD1A antibody (orb1239525) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of JMJD1A in Rat Liver Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat liver cells labeling JMJD1A with orb1239525 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
KDM3A Antibody (orb1239525)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Free Secondary Antibody (20 ul)0/0
Please add an antibody product to your cart first.
Usually dispatched within 5-10 working days