K777

SKU: orb1707146

Description

K777 is a potent, orally available irreversible cysteine protease inhibitor targeting cruzain and cathepsins B/L. It also inhibits CYP3A4 and CCR4, and it blocks the cellular entry of pseudoviruses like EBOV and SARS-CoV in vitro, supporting research in parasitology and virology.

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research, Pharmacology & Drug Discovery, Protein Biochemistry

Images & Validation

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Key Properties

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CAS Number	233277-99-1
MW	574.73
Purity	99.14%
Formula	C₃₂H₃₈N₄O₄S
SMILES	CN1CCN(CC1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCc1ccccc1)\C=C\S(=O)(=O)c1ccccc1
Target	SARS-CoV,Virus Protease,Parasite,Cysteine Protease,CCR,Cytochromes P450
Solubility	DMSO:90 mg/mL (156.6 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (5.74 mM)

Bioactivity

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Target IC50	CYP3A4:60 nM (IC50)
In Vivo	In C57BL/6 IFN-γR-KO mice, K777 ( 35-105 mg/kg; p.o.) rescues mice from otherwise lethal infections.
In Vitro	K777 is a broad-spectrum antiviral and inhibits SARS-CoV, HCoV-229E, NL63, MERS-CoV, EBOV, SUDV, TAFV, RESTV, BEBOV, MARV and Nipah pseudovirus entry with IC50 values of 0.68 nM, 1.48 nM, 6.78 nM, 46.12 nM, 0.87 nM, 1.14 nM, 2.26 nM, 3.37 nM, 5.91 nM, 1.9 nM and 0.42 nM, respectively. K777 alone demonstrates up to 70% inhibition of 229E-S-mediated transduction in TMPRSS2 expressing cells. Simultaneous treatment with Camostat and K777 increases inhibition to ~ 90%. K777 inhibits both CCL17 binding and CCL17-induced chemotaxis in Hut78 cells with IC50s of 57 and 8.9 nM, respectively. The K777-mediated inhibition of chemotaxis is potent even in the presence of a 10-fold higher concentration of CCL17. K777 induces CCR4 internalization with a 50% reduction of cell surface CCR4. Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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K777, K-777, K 777, CCR4, Cysteine Protease, Cysteine protease, CysteineProtease, CYP3A4, APC3316, APC-3316, APC 3316

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID

9851116 ↗

Key Properties

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No computed properties available.

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K777 (orb1707146)

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K777

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

Quality Guarantee

Compound Identifiers

Key Properties

Documents Download

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Protocol Information

K777 (orb1707146)

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