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Recombinant Kappa Light Chain Antibody

SKU: orb2641247

Description

This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Images & Validation

Tested ApplicationsIHC-P
Dilution RangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
ReactivityHuman
Application Notes
The optimal dilution of the recombinant Kappa Light Chain antibody for each application should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityRecombinant
IsotypeMouse IgG1, kappa
Clone No.rL1C1
ImmunogenRecombinant human Ig kappa chain was used as the immunogen for this recombinant Kappa Light Chain antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Recombinant Kappa Light Chain Antibody

IHC testing of FFPE human tonsil tissue with recombinant Kappa Light Chain antibody (clone rL1C1). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Recombinant Kappa Light Chain Antibody

SDS-PAGE analysis of purified, BSA-free recombinant Kappa Light Chain antibody (clone rL1C1) as confirmation of integrity and purity.

Recombinant Kappa Light Chain Antibody

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using recombinant Kappa Light Chain antibody (clone rL1C1). These results demonstrate the foremost specificity of the rL1C1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol

Recombinant Kappa Light Chain Antibody (orb2641247)

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100 μg
$ 750.00
DispatchUsually dispatched within 5-10 working days
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