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KDEL Antibody (RPE)
SKU: orb413475
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB (1:1000); IHC (1:25) |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 2C1 |
| Immunogen | KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Target | KDEL |
| Purification | Protein G Purified |
| Conjugation | RPE |
Storage & Handling
−| Storage | Conjugated antibodies should be stored according to the product label |
|---|---|
| Buffer/Preservatives | 95.46mM Phosphate, 2.48mM MES and 2mM EDTA |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−KDEL, K-D-E-L, Lys-Asp-Glu-Leu, Lys-Asp-Glu-Leu (KDEL), Lysine-aspartic acid-glutamate-leucine
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Immunohistochemistry analysis using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1. Tissue: kidney. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) KDEL Antibody (D) Composite.
Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control. Lane 4: Anti-PDI control. Lane 5: Anti-Calreticulin control. Lane 6: Anti-GRP78 control. Lane 7: Anti-GRP94 control. Load: 10 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.
Quick Database Links
Gene Symbol
KDEL
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
KDEL Antibody (RPE) (orb413475)
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