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KDM4B Recombinant Rabbit Monoclonal Antibody

SKU: orb1172985

Description

KDM4B Recombinant Rabbit Monoclonal Antibody

Images & Validation

Tested ApplicationsFC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
ReactivityHuman, Mouse, Rat
Predicted ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG
Clone No.G8C10
TargetKDM4B
Molecular Weight122 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

KDM4B_HUMAN; Lysine-specific demethylase 4B; JHDM3B; JMJD2B; KIAA0876; EC:1.14.11.66; JmjC domain-containing histone demethylation protein 3B; Jumonji domain-containing protein 2B; [histone H3]-trimethyl-L-lysine(9) demethylase 4B; lysine demethylase 4B; MRD65; TDRD14B

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

KDM4B Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: T47D, 2: LNCap, 3: K562, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 122 kDa, Observed MW: 150 kDa.

KDM4B Recombinant Rabbit Monoclonal Antibody

Cell line: K562, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1172985.

KDM4B Recombinant Rabbit Monoclonal Antibody

Cell line: K562, Fixative: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1172985.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
FC
Flow Cytometry
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IF
Immunofluorescence
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ICC
Immunocytochemistry
View Protocol

KDM4B Recombinant Rabbit Monoclonal Antibody (orb1172985)

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Available Sizes

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25 μl
$ 170.00
50 μl
$ 320.00
100 μl
$ 460.00
DispatchUsually dispatched within 5-10 working days
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