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Ki-67 (Proliferating Cell Marker) Antibody
SKU: orb751175
Description
Images & Validation
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| Tested Applications | FC, IF, IHC |
|---|---|
| Dilution range | Flow Cytometry (5ul per test per million cells); Immunofluorescence (2-4ug/ml at RT);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT);,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | MKI67/2465 |
| Immunogen | Recombinant fragment (around aa 2293-2478) of human Ki67 protein (exact sequence is proprietary) |
| Target | MKI67 |
| Source/Expression System | Bioreactor |
| Molecular Weight | 345kDa and 395kDa |
| Purification | Protein A |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Buffer/Preservatives | Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide. |
| Concentration | Purified Ab conjugated to Biotin |
| Disclaimer | For research use only |
Alternative Names
−Anti-KI-67 antibody, anti-Ki67 antibody, anti-KI-67 Antigen (KIA) antibody, anti-MKI67 antibody, anti-Proliferation related Ki-67 antigen antibody
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SDS-PAGE Analysis Purified Ki67 Mouse Monoclonal Antibody (MKI67/2465) (unconjugated) Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human tonsil stained with Biotin-conjugated Ki67 Mouse Monoclonal Antibody (MKI67/2465).
Analysis of Protein Array containing more than 19000 full-length human proteins using Ki67-Monospecific Mouse Monoclonal Antibody (MKI67/2465). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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Ki-67 (Proliferating Cell Marker) Antibody (orb751175)
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