KIFC1 Rabbit Polyclonal Antibody

SKU: orb101014

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Description

Research Area

Cell Biology, Kinesin-like Proteins, Motor Proteins

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Rat
Predicted Reactivity	Bovine, Equine, Mouse, Porcine

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human KIFC1 (401-500/673aa)
Target	KIFC1
Molecular Weight	74 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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HSET KNSL2; KIF C1; KIFC 1; KIFC1; KIFC1_HUMAN; Kinesin family member C1; Kinesin like 2; Kinesin like protein 2; Kinesin like protein KIFC1; Kinesin related protein HSET; Kinesin-like protein 2; Kinesin-like protein KIFC1; Kinesin-related protein HSET; KNSL 2; KNSL2; Mitotic kinesin like protein 2; MKLP 2; MKLP2; RAB6 KIFL; Rabkinesin 6.

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Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (KIFC1) Polyclonal Antibody, Unconjugated (orb101014) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (KIFC1) Polyclonal Antibody, Unconjugated (orb101014) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A549 Cell (Human) Lysate at 40 ug, Primary: Anti-KIFC1 (orb101014) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 74 kD, Observed band size: 74 kD.

Sample: Raji Cell (Human) Lysate at 40 ug, Primary: Anti-KIFC1 (orb101014) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 74 kD, Observed band size: 74 kD.

Quick Database Links

Gene Symbol

KIFC1

UniProt

Q9BW19

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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