Kir2.1 Rabbit Polyclonal Antibody

SKU: orb184788

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Description

Images & Validation

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Item 1 of 4

Tested Applications	FC, WB
Dilution range	WB=1:500-2000, Flow-Cyt=1μg/Test
Reactivity	Mouse, Rat
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Kir2.1 (31-130/427aa)
Target	KCNJ2
Molecular Weight	48 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Cardiac inward rectifier potassium channel; HHBIRK 1; HHBIRK1; HHIRK1; HIRK 1; hIRK1; Inward rectifier K; inwardly rectifying subfamily J member 2; IRK 1; IRK2_HUMAN; IRK1; KCNJ2; KIR2.1; LQT7; Potassium channel; Potassium channel inwardly rectifying subfamily J member 2; Potassium inwardly rectifying channel J2; Potassium inwardly rectifying channel subfamily J member 2; SQT 3; SQT3.

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Blank control (blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Kir2.1 antibody (orb184788), dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Bone (Mouse) Lysate at 40 ug, Primary: Anti-Kir2.1 at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.

Sample: heart (Mouse) Lysate at 40 ug, Primary: Anti-Kir2.1 (orb184788) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Kir2.1 at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.

Quick Database Links

Gene Symbol

KCNJ2

UniProt

P63252

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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