CD161 Antibody / KLRB1

SKU: orb2635883

Description

Natural killer (NK) and T cells express a superfamily of proteins with structural features of C-type lectins. T cells bearing natural killer receptors (NKRs) such as CD94 and CD161 are present in psoriasis. CD161 mediates NK cell activation and functions as an activating receptor. CD161 is a prototypic marker of NK cells, although it is also found on a subset of CD8+ T cells. The expression of NK receptors on CD8+ T cells can be considered a marker of cytotoxic effector T cells that are expanded in vivo after antigenic activation leading to extensive proliferation. The transcription, mRNA accumulation, and surface expression of CD161, a molecule involved in triggering cytotoxicity, is specifically upregulated by IL-12.

Images & Validation

−

Item 1 of 3

Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the CD161 antibody should be determined by the researcher.

Key Properties

−

Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	KLRB1/8910
Immunogen	A recombinant fragment (within amino acids 1-225) of human KLRB1 protein was used as the immunogen for the CD161 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Western blot testing of human spleen tissue lysate with CD161 antibody. Predicted molecular weight ~25 kDa but may be observed at higher molecular weights due to glycosylation.

IHC staining of FFPE human prostate tissue with CD161 antibody (clone KLRB1/8910). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free CD161 antibody (clone KLRB1/8910) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q12918

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

IHC-P

Immunohistochemistry Paraffin

View Protocol

Find More Protocols