[KO Validated] Bax Rabbit pAb Discontinued

SKU: orb129529

This product can be custom made

Description

Rabbit polyclonal antibody to BAX

Research Area

Apoptotic, Cancer, Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways

Images & Validation

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Item 1 of 9

Tested Applications	ELISA, IF, IHC-P, IP, WB
Dilution Range	WB: 1:500-2000, IHC-P: 1:50-100
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).
Molecular Weight	21kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti-Apoptosis regulator BAX antibody, anti- BAX antibody, anti- BAX-protein antibody, anti- BAX_HUMAN antibody, anti- BAXA antibody, anti- BAXdelta2G9 antibody, anti- BAXdelta2G9omega antibody, anti- BAXdelta2omega antibody, anti- omega antibody, anti- transcript variant delta2 antibody, anti- membrane isoform alpha antibody

Quality Guarantee

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Immunofluorescence analysis of HeLa cells using [KO Validated] Bax Rabbit pAb (orb129529) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HepG2 cells using [KO Validated] Bax Rabbit pAb (orb129529) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of MCF7 cells using [KO Validated] Bax Rabbit pAb (orb129529) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Bax Rabbit pAb (orb129529) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using [KO Validated] Bax Rabbit pAb (orb129529) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using [KO Validated] Bax Rabbit pAb (orb129529) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot analysis of lysates from Mouse brain, using [KO Validated] Bax Rabbit pAb (orb129529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Western blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells, using [KO Validated] Bax Rabbit pAb (orb129529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western blot analysis of various lysates using [KO Validated] Bax Rabbit pAb (orb129529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.