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KRT1 Antibody (Center)

SKU: orb1927782

Description

KRT1 Antibody (Center)

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsFC, IHC-P, WB
Dilution RangeWB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50
ReactivityHuman

Key Properties

HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
Molecular Weight66039 Da
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

KRTA
Quality Guarantee

Quality Guarantee

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KRT1 Antibody (Center)

Western blot analysis of KRT1 Antibody (Center) in MCF-7 cell line lysates (35 ug/lane). KRT1 (arrow) was detected using the purified Pab.

KRT1 Antibody (Center)

KRT1 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

KRT1 Antibody (Center)

All lanes: Anti-KRT1 Antibody (Center) at 1:1000 dilution + 293 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 66KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

KRT1 Antibody (Center)

KRT1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRT1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FC
Flow Cytometry
View Protocol

KRT1 Antibody (Center) (orb1927782)

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50 μl
$ 170.00
100 μl
$ 310.00
DispatchUsually dispatched within 5-10 working days
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