Recombinant Cytokeratin 7 Antibody

SKU: orb606505

Description

CK7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Expressed in cultured epidermal, bronchial and mesothelial cells. [UniProt]

Research Area

Epigenetics, Infectious Diseases

Images & Validation

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Tested Applications	FACS, IHC-P, WB
Dilution range	Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Reactivity	Human
Application Notes	The stated application concentrations are suggested starting amounts. Optimal dilution of the recombinant Cytokeratin 7 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rOV-TL12/30
Immunogen	OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the recombinant Cytokeratin 7 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC testing of FFPE human endometrial carcinoma with recombinant Cytokeratin 7 antibody (clone rOV-TL12/30). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 7 antibody (clone rOV-TL12/30) as confirmation of integrity and purity.

Western blot testing of human HeLa cell lysate with recombinant Cytokeratin 7 antibody (clone rOV-TL12/30).

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Cytokeratin 7 antibody (clone rOV-TL12/30); Red = isotype control, Blue = recombinant Cytokeratin 7 antibody.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using recombinant Cytokeratin 7 antibody (clone rOV-TL12/30). These results demonstrate the foremost specificity of the rOV-TL12/30 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Quick Database Links

UniProt

P08729

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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