Recombinant Cytokeratin 8 Antibody / Rabbit Monoclonal

SKU: orb2640541

Description

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium.

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Tested Applications	IHC-P, WB
Dilution range	Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Reactivity	Human
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Cytokeratin 8 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	KRT8/2174R
Immunogen	A cytoskeletal preparation from human HeLa cells was used as the immunogen for this recombinant Cytokeratin 8 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Recombinant Cytokeratin 8 Antibody / Rabbit Monoclonal

Western blot testing of human HCT-116 cell lysate with recombinant Cytokeratin 8 antibody (clone KRT8/2174R).

SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 8 antibody (clone KRT8/2174R) as confirmation of integrity and purity.

IHC testing of FFPE human small intestine tissue with recombinant Cytokeratin 8 antibody (clone KRT8/2174R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

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UniProt

P05787

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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