CDH16 Antibody (Biotin)

SKU: orb750824

Description

Mouse monoclonal antibody against Cadherin-17 conjugated to Biotin

Research Area

Kidney & Urological Research

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA (For coating, order Ab without BSA);Western Blot (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Reactivity	Human
Application Notes	Positive Control: Normal kidney or renal cell carcinoma

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	CDH16/2125
Immunogen	Recombinant fragment (around aa 371-507) of human CDH16 protein (exact sequence is proprietary)
Target	CDH16
Source/Expression System	Bioreactor
Molecular Weight	130kDa
Purification	Protein G
Conjugation	Biotin

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide. Contact us if you require this Ab in a different format.
Buffer/Preservatives	Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Concentration	Purified Ab conjugated to Biotin
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-Cadherin-16 (CDH16) antibody, anti-Kidney-specific cadherin antibody, anti-Ksp-cadherin antibody antibody

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Analysis of Protein Array containing more than 19000 full-length human proteins using CDH16-Monospecific Mouse Monoclonal Antibody (CDH16/2125). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human kidney stained with Biotin-conjugated CDH16 Mouse Monoclonal Antibody (CDH16/2125).

SDS-PAGE Analysis Purified CDH16-Monospecific Mouse Monoclonal Antibody (CDH16/2125). Confirmation of Integrity and Purity of Antibody.

Quick Database Links

Gene Symbol

CDH16

UniProt

O75309

NCBI Gene ID

1014

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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