Lamin B1 Rabbit Polyclonal Antibody

SKU: orb1294412

Description

Rabbit polyclonal antibody to Lamin B1

Images & Validation

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Item 1 of 3

Tested Applications	IF, WB
Dilution Range	Western Blot 1:1000-1:3000, Immunofluorescence 1:200-1:500
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / encompassing a sequence within the C-terminus region.
Target	Lamin B1
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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A549 cells'cytoplasmic and nuclear proteins extracted by 30-min. A. SDS-PAGE gel shows the profiles of extracted cytoplasmic proteins and nuclear proteins. B. The proteins from the gel were transferred to nitrocellulose membrane and probed with Lamin B1 antibody, Histone H3 antibody (nuclear marker) and GAPDH antibody (cytoplasmic marker). Nuclear Protein Extraction Kit.

All lanes: Anti-Lamin B1 antibody - at 1/2000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294412 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294412 anti-Lamin B1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

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Gene Symbol

Lamin B1

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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