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LAMP2 Mouse Monoclonal Antibody
SKU: orb1973123
Description
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500-1:1000, Immunofluorescence 1:100-1:300, Immunohistochemistry (Paraffin, Frozen) 1:50-1:150 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Target | LAMP2 / CD107b |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Disclaimer | For research use only |
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Anti-LAMP2 / CD107b monoclonal antibody at 1/500 dilution, Lysates at 50 µg per lane, This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1973123 overnight at 4°C.
Human acute myeloid leukemia cell line KG1a was fixed, permeabilized, and stained with LAMP2 / CD107b monoclonal antibody orb1973123: 1-50.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1973123 anti-LAMP2 / CD107b antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling LAMP2 / CD107b with orb1973123 at 1/100.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling LAMP2 / CD107b with orb1973123 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling LAMP2 / CD107b with orb1973123 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Quick Database Links
Gene Symbol
LAMP2 / CD107b
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
LAMP2 Mouse Monoclonal Antibody (orb1973123)
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