Leupeptin Hemisulfate

SKU: orb1300655

Description

Research Area

Infectious Disease & Virology, Protein Biochemistry

Images & Validation

−

Key Properties

−

CAS Number	103476-89-7
MW	475.59
Purity	99.17%
Formula	C₂₀H₃₈N₆O₄·1/2H₂SO₄
SMILES	OS(O)(=O)=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C(N)N)C=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C(N)N)C=O
Target	Cysteine Protease,Virus Protease,Serine Protease
Solubility	H2O:87 mg/mL (182.93 mM);DMSO:60 mg/mL (126.16 mM);Ethanol:88 mg/mL (185.03 mM)

Bioactivity

−

Target IC50	Huh7 cells (SARS-CoV-2):39.29 µM (EC50)\|SARS-CoV-2 (Vero cells):42.34 µM (EC50)\|Human coronavirus strain 229E:0.4 µg/mL
In Vivo	METHODS: To determine macrophage autophagic flux, Leupeptin hemisulfate (9-40 mg/kg in 0.5 mL PBS) was administered to C57BL/6NCrl mice by single intraperitoneal injection. RESULTS: Leupeptin hemisulfate treatment resulted in the highest accumulation of LC3b in the liver and the lowest in the spleen. LC3a, ATG8/LC3b homologs, and the LC3b-interacting protein p62 were degraded with kinetics similar to those of LC3b. METHODS: To examine whether cold activates autophagic flux, Leupeptin hemisulfate (40 mg/kg) was administered intraperitoneally to C57B6 mice in a single injection, and then the mice were cold-exposed at 4 ℃ for 1 h. The mice were then exposed to cold for 1 h. RESULTS: LC3-II flux in brown fat was increased >2-fold in cold-exposed mice. Read more...
In Vitro	METHODS: SARS-CoV-2 infected Vero cells were treated with Leupeptin hemisulfate (0.06-200 µM) for 72 h, and the viral RNA level was detected by RT-PCR. RESULTS: Leupeptin hemisulfate inhibited SARS-CoV-2 RNA levels in Vero cells with an EC50 value of 42.34 µM. METHODS: SARS-CoV-2 pseudovirus-infected Huh7 cells were treated with Leupeptin hemisulfate (0.1-100 µM) for 24 h. Pseudovirus infection was detected by luciferase activity assay. RESULTS: Leupeptin hemisulfate inhibited pseudovirus infection in Vero cells with an EC50 value of 39.29 µM. Read more...

Storage & Handling

−

Storage	keep away from moisture,store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Cysteine protease, Cysteine Protease, CysteineProtease, Cathepsin, Inhibitor, Leupeptin, Leupeptin Hemisulfate, inhibit, Serine Protease, SerineProtease

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID

2733491 ↗

Key Properties

−

No computed properties available.

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols