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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Bovine |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This LGR5 (GPR49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-473 amino acids from the Central region of human LGR5 (GPR49). |
| Target | LGR5 |
| Molecular Weight | 100 kDa |
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67
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Western blot analysis of in K562 cell line lysates (35 ug/lane).
Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow) (Kindly offered by Dr. Li).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
LGR5 Antibody (orb1264632)
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