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LGR5/GPR49 Recombinant Rabbit Monoclonal Antibody
SKU: orb2563049
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Description
Research Area
G Protein-Coupled Receptors, GPCR Signaling Pathway, Molecular Biology, Surface Molecules
Images & Validation
−Item 1 of 2
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB=1:500-2000, Flow-Cyt=1:50-100 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 8H13 |
| Immunogen | A synthesized peptide derived from human LGR5 (818-907aa) |
| Target | LGR5 |
| Molecular Weight | 98 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−G protein coupled receptor 49; G-protein coupled receptor 49; G-protein coupled receptor 67; GPCR GPR49; G protein coupled receptor 67; FEX; g protein-coupled receptor fex; G-protein coupled receptor HG38; GPR 49; GPR 67; GPR49; GPR67; Leucine rich repeat containing G protein coupled receptor 5; HG 38; HG38; LGR5_HUMAN; MGC117008; Leucine-rich repeat-containing G-protein coupled receptor 5; LGR 5; Orphan G protein coupled receptor HG38.
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25 ug total protein per lane of various lysates probed with LGR5/GPR49 monoclonal antibody, unconjugated (orb2563049) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-LGR5/GPR49 antibody (orb2563049): 1 µg/10^6 cells, Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 µg/Test. Isotype Control (orange): Rabbit IgG (orb570250). Blank control (black): PBS. Acquisition of 20000 events was performed.
Quick Database Links
Gene Symbol
LGR5
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
LGR5/GPR49 Recombinant Rabbit Monoclonal Antibody (orb2563049)
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