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LIF Recombinant Protein
Description
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | ED50 is less than 0.01 ng/ml as determined by the M1 cell differentiation assay.Specific Activity of 1.0 x 108 IU/mg. |
| Target | LIF |
| Tag | Tag Free |
| Molecular Weight | 19.7 kD |
| Protein Sequence | Ser23-Phe202 |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 0.02% Tween 20, pH 7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human LIF Protein, hFc Tag [orb1290937]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 46 kDa after removal of the signal peptide. The apparent molecular mass of LIF-His is approximately 55-70 kDa due to glycosylation.
Mammalian
50 μg, 10 μg, 100 μgRecombinant Human CD118/LIFR Protein, N-His [orb2968547]
>90% as determined by SDS-PAGE.
18.19 kDa
1 mg, 100 μg, 50 μgRecombinant Human LIF Protein, N-His [orb2969124]
>90% as determined by SDS-PAGE.
21.93 kDa
1 mg, 100 μg, 50 μg

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LIF Recombinant Protein (orb1229847)
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