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Description
Research Area
Immunology & Inflammation
Images & Validation
−
| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | ED50 is less than 0.01 ng/ml as determined by the M1 cell differentiation assay.Specific Activity of 1.0 x 108 IU/mg. |
| Target | LIF |
| Tag | Tag Free |
| MW | 19.7 kD |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| Protein Sequence | Ser23-Phe202 |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 0.02% Tween 20, pH 7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Leukemia Inhibitory Factor, LIF, Differentiation-Stimulating Factor, D Factor, Melanoma-Derived LPL Inhibitor, MLPLI, Emfilermin, HILDA
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LIF Recombinant Protein (orb1229847)
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