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LIF Recombinant Protein
Description
Research Area
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Murine LIF is fully biologically active when compared to standards. The ED50 as determined by the M1 cell differentiation assay is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg. |
| Target | Lif |
| Protein Sequence | SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF |
| Purity | ≥ 98% by SDS-PAGE gel and HPLC analyses. |
Storage & Handling
−| Storage | The recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human LIF Protein, hFc Tag [orb1290937]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 46 kDa after removal of the signal peptide. The apparent molecular mass of LIF-His is approximately 55-70 kDa due to glycosylation.
Mammalian
50 μg, 10 μg, 100 μgRecombinant Human CD118/LIFR Protein, N-His [orb2968547]
>90% as determined by SDS-PAGE.
18.19 kDa
1 mg, 100 μg, 50 μgRecombinant Human LIF Protein, N-His [orb2969124]
>90% as determined by SDS-PAGE.
21.93 kDa
1 mg, 100 μg, 50 μg

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Protocol Information
LIF Recombinant Protein (orb1230434)
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