Llama IgG Whole Molecule Antibody

SKU: orb346447

Description

Research Area

Protein Biochemistry

Images & Validation

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Item 1 of 1

Tested Applications	SDS-PAGE
Application Notes	Llama IgG whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, flow cytometry, and ELISA experiments. Specific conditions for reactivity should be optimized by the end user. Llama IgG whole molecule buffered in 0.015 M Potassium Phosphate, 0.01125 M Sodium Chloride, pH 7.2 and 25% glycerol. Read more...

Key Properties

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Source	Llama
Biological Origin	Llama
Isotype	IgG
Purity	Llama IgG has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. This product contains equal amounts of IgG1, IgG2 and IgG3. Coomassie stained SDS-PAGE of non-reduced llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain); non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa; non-reduced llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide; Buffer: See application note.
Concentration	1.40 mg/mL
Expiration Date	12 months from date of receipt.
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

Alternative Names

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Immunoglobulin G from Llama, IgG from Llama, Llama IgG, Llama IgG Whole Molecule

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Purity:	Llama IgG has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced unconjugated llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain); non-reduced unconjugated llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa; non-reduced unconjugated llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa. Fluorescein Conjugated Llama IgG Whole molecule was proven by dot blot.
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SDS PAGE Results of Llama IgG Whole Molecule. Lane 1: Llama IgG Reduced [1.0 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Llama IgG Non-Reduced [1.0 µg]. 4-20% Gel, Coomassie Stained.

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Protocol Information

SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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