LRP1 Antibody (HRP)

SKU: orb535887

Description

Rabbit polyclonal antibody against LRP-1 conjugated to HRP

Images & Validation

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Tested Applications	IHC, WB
Dilution range	WB (1:1000); IHC (1:50)
Reactivity	Human, Mouse, Rat
Application Notes	A 1:1000 dilution of SPC-785 was sufficient for detection of LRP-1 in 15 µg of mouse Brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide from the N-terminal of Human Low-density lipoprotein receptor-related protein 1 (aa. 160-260)
Target	LRP1
Molecular Weight	80 kDa
Purification	Peptide Affinity Purified
Conjugation	HRP

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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LRP1, LRP-1, LRP-85, LRP-515, Alpha-2-macroglobulin receptor, A2MR, Apolipoprotein E receptor, Prolow-density lipoprotein receptor-related protein 1, CD91, LRPICD, APOER, APR

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Immunohistochemistry analysis using Rabbit Anti-LRP1 Polyclonal Antibody. Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-LRP1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Mouse, Rat Brain showing detection of 504.6 kDa LRP-1 protein using Rabbit Anti-LRP-1 Polyclonal Antibody. Load: 15 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-LRP-1 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 504.6 kDa. Other Band (s): 80 kDa.

Quick Database Links

Gene Symbol

LRP1

UniProt

Q07954

NCBI Gene ID

4035

RefSeq (Protein):NP_002323.2

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002323.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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