Lysine Acetylated Antibody Biotin Conjugated

SKU: orb420484

Description

Lysine Acetylated antibody (Biotin)

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:5,000, WB: 1:1,000-1:5,000
Application Notes	This affinity-purified antibody is suitable for use in ELISA and western blotting assays. The antibody reacts specifically with acetylated lysine residues. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with acetylated lysine conjugated KLH.
Purity	Anti-AcK was prepared from monospecific antiserum by immunoaffinity chromatography using acetylated lysine peptide coupled to agarose followed by conjugation to biotin and subsequent purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin. Reactivity is specific for acetylated lysine.
Conjugation	Biotin

Storage & Handling

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Storage	Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	None
Concentration	1 mg/mL
Disclaimer	For research use only

Alternative Names

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acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody

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Lysine Acetylated Antibody Biotin Conjugated

Biorbyt's Affinity Purified anti-Acetylated Lysine (AcK) antibody is shown to detect acetylated histone in TSA-treated mouse spleen cell lysate (Panel A); control (left lane) and TSA-treated mouse spleen cell lysate (right lane) in panel B; and in acetylated BSA loaded as indicated (panel C).

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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