MLKL Rabbit Polyclonal Antibody

SKU: orb1935968

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

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Item 1 of 5

Tested Applications	IHC-P, WB
Dilution Range	IHC-P: 1~400, WB: 1:2000, WB: 1:2000, WB: 1:1000, WB: 1:2000
Reactivity	Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	RB34842
Target	This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl.
Molecular Weight	54317 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Mixed lineage kinase domain-like protein, Mlkl {ECO:0000312|EMBL:AAH237551, ECO:0000312|MGI:MGI:1921818}

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Western blot analysis of lysate from mouse lung tissue lysate, using Mlkl Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Immunohistochemical analysis of paraffin-embedded Human tonsil section using M Mlkl antibody. Diluted at 1~400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-M Mlkl Antibody (C-term) at 1:2000 dilution. Lane 1: NIH/3T3 whole cell lysate. Lane 2: mouse spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 53KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Mlkl Antibody (C-term) at 1:2000 dilution. Lane 1: mouse testis lysates. Lane 2: mouse liver lysates. Lane 3: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Mlkl Antibody (C-term) at 1:2000 dilution. Lane 1: mouse lung lysates. Lane 2: NIH/3T3 whole cell lysates. Lane 3: mouse liver lysates. Lane 4: mouse testis whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl.

UniProt

Q9D2Y4

RefSeq (Protein):NP_083281.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_083281.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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