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Description
Research Area
Cancer Research, Cell Biology, Cell Cycle, Immunology
Images & Validation
−Item 1 of 3
| Tested Applications | WB |
|---|---|
| Dilution Range | WB=1:500-2000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | B8E8 |
| Immunogen | Recombinant human Mad2L1 protein |
| Target | MAD2L1 |
| Molecular Weight | 23 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HSMAD2; MAD2; MD2L1_HUMAN; MAD2L1; Mitotic arrest deficient 2-like protein 1 (MAD2-like protein 1); mitotic arrest deficient 2 like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
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MAD2L1BP Recombinant Rabbit Monoclonal Antibody [orb2562839]
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50 μl, 100 μl, 25 μl
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A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Mad2L1) monoclonal Antibody, Unconjugated (orb704306) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
ICC staining Mad2L1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Mad2L1 on different cell lysates using anti-Mad2L1 antibody at 1/500 dilution. Positive control: Lane 1: K562, Lane 2: 293T, Lane 3: SH-SY5Y.
Quick Database Links
Gene Symbol
MAD2L1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Mad2L1 Recombinant Rabbit Monoclonal Antibody (orb704306)
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