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Description
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution Range | IF: 1:25, WB: 1:1000, IHC-P: 1:25 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This MAD2L2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 198-231 amino acids from the C-terminal region of human MAD2L2. Antigen Region: 198-231 aa. |
| Target | MAD2L2 |
| Molecular Weight | 24334 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Mitotic spindle assembly checkpoint protein MAD2B, Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, REV7 homolog, hREV7, MAD2L2, MAD2B, REV7
Similar Products
−- Item 1 of 3
MAD2L2 Antibody (C-term) [orb1927098]
IF, IHC-P, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl
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Western blot analysis of lysates from Hela, K562 cell line (from left to right), using MAD2L2 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. kidney section using MAD2L2 Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Fluorescent image of Hela cells stained with MAD2L2 Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Quick Database Links
Gene Symbol
MAD2L2
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
MAD2L2 Antibody (C-term) (orb1788459)
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