Recombinant MVP Antibody / Major Vault Protein

SKU: orb1825368

Description

Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

Images & Validation

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Tested Applications	IHC-P, WB
Dilution range	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant MVP antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	r1032
Immunogen	Proteins precipitated from human breast cancer MCF-7 cells were used as the immunogen for the recombinant MVP antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Recombinant MVP Antibody / Major Vault Protein

IHC staining of FFPE human prostate tissue with recombinant MVP antibody (clone r1032). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human U-87, A549 and SK-BR-3 cell lysate with recombinant MVP antibody (clone r1032). Expected molecular weight: 104-110 kDa.

Quick Database Links

UniProt

Q14764

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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