Recombinant Mammaglobin A Antibody / SCGB2A2

SKU: orb2642205

Description

SCGB2A2 is a member of the superfamily of secretoglobins, a group of small dimeric secreted and sometimes glycosylated proteins. Expressed mainly in mucosa, secretoglobins seem to be involved in cell signalling, immune response, and chemotaxis, and may also serve as transporters for steroid hormones in humans. SCGB2A2 expression is highly specific of mammary tissue, and is increasingly used for identification and detection of disseminated breast cancer cells.

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Tested Applications	IHC-P, WB
Dilution Range	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Reactivity	Human
Application Notes	The stated application concentrations are suggested starting points. Titration of the recombinant Mammaglobin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	MMGA-1R
Immunogen	Full length recombinant human protein was used as the immunogen for the recombinant Mammaglobin A antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Mammaglobin A Antibody / SCGB2A2

Western blot testing of human MCF-7 cell lysate with recombinant Mammaglobin A antibody (clone MMGA-1R). Expected molecular weight: 10-21 kDa depending on level of glycosylation.

IHC testing of FFPE human breast carcinoma stained with recombinant Mammaglobin A antibody (clone MMGA-1R). Required HIER: boiling tissue sections in 10 mM citrate buffer, pH6, for 10-20 min followed by cooling prior to testing.

Quick Database Links

UniProt

Q13296

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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