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MAP4K3 Antibody
SKU: orb1939368
Description
Research Area
Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:500-1000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM,k |
| Molecular Weight | 101316 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−RAB8IPL1
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MAP4K3 western blot analysis in A2058 cell line lysates (35 μg/lane). This demonstrates the MAP4K3 antibody detected the MAP4K3 protein (arrow).
Western blot analysis of lysate from MCF-7 cell line, using MAP4K3. diluted at 1:1000. A goat anti-mouse IgM H&L (HRP) at 1:10000 dilution was used as the secondary Antibody. Lysate at 20 μg.
Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using MAP4K3 Antibody. diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysates from HepG2, MCF-7 cell line (from left to right), using MAP4K3 Antibody. diluted at 1:1000 at each lane. A goat anti-mouse IgM H&L (HRP) at 1:3000 dilution was used as the secondary Antibody. Lysates at 35 μg per lane.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
MAP4K3 Antibody (orb1939368)
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