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Featured
KO/KD
Validated
Validated
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, KO/KD Validated, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-424 of human MAPK9 (NP_002743.3). |
| Target | MAPK9 |
| Molecular Weight | Observed: 54kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−MAPK9, stress-activated protein kinase JNK2, SAPK, PRKM9, p54aSAPK, p54a, OTTHUMP00000161543, OTTHUMP00000161542, mitogen-activated protein kinase 9, MAPK 9, MAP kinase 9, Jun kinase, JNK-55, JNK2BETA, JNK2B, JNK2ALPHA, JNK2A, JNK2, c-Jun N-terminal kinase 2, c-Jun kinase 2, SAPK1A
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Western blot analysis of extracts of various cell lines, using MAPK9 antibody (orb1274518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and MAPK9 knockout (KO) 293T cells, using MAPK9 antibody (orb1274518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human breast cancer using MAPK9 antibody (orb1274518) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MAPK9 antibody (orb1274518) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using MAPK9 antibody (orb1274518). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
MAPK9
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
MAPK9 Antibody (orb1274518)
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