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Description
Images & Validation
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Item 1 of 8
| Tested Applications | ELISA, FC, ICC, IF, WB |
|---|---|
| Dilution Range | WB:1:10,000-1:30,000, IF:1:200-1:1,000, ELISA:1:20,000-1:50,000, FC: 2 ug/test |
| Reactivity | Other |
| Application Notes |
Key Properties
−| Host | Gallus |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgY |
| Immunogen | Antigen: Affinity purified recombinant Red Fluorescent Protein (mCherry). Antigen Sequence: MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK |
| Target | Red Fluorescent Protein |
| Molecular Weight | 27 kDa |
| Purification | Epitope affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Polyclonal antibody supplied as a 100 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. Affinity purified using the immunogen immobilized on a solid support; Sample purity >95% (SDS-PAGE). |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.
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Quick Database Links
Gene Symbol
Red Fluorescent Protein
Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)