ME1 Mouse Monoclonal Antibody

SKU: orb1973116

Description

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:500 – 1:1000, Immunofluorescence 1:100 – 1:200, Immunohistochemistry (Paraffin) 1:50 – 1:100
Reactivity	Human, Mouse, Rat
Application Notes	Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Target	ME1 / malic enzyme 1
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Phosphate-buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Immunofluorescence of cell. Sample: HeLa, Green: ME1 / malic enzyme 1, monoclonal antibody (orb1973116): 1-100.

orb1973116 ME1 / malic enzyme 1, monoclonal antibody at 1/500 dilution. Lysates at 35 µg per lane. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1973116 overnight at 4°C.

Quick Database Links

Gene Symbol

ME1 / malic enzyme 1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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