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MerTK Antibody

SKU: orb639575

Description

MerTK, also called c-Mer, is a member of the Mer/Axl/Tyro3 receptor kinase family. It is a 984 residue transmembrane protein made up of one tyrosine kinase domain, two Fibronectin type-III domains and two immunoglobulinlike C2-type domains. MerTK is the mammalian ortholog of the chicken retroviral oncogene product v-Eyk. This protein plays a critical role in macrophage activation, platelet aggregation, clot stability and the efficient removal of apoptotic cells. Specifically, MerTK acts as a signaling molecule, triggering outer segment ingestion in the retinal pigment epithelium (RPE) phagocytic process. Evidence suggests that MerTK signals via interaction with phosphatidylinositol-specific phospholipase C 2). When the gene encoding for MerTK is mutated, the RPE phagocytosis pathway is disrupted and autosomal recessive retinitis pigmentosa (RP) may result, leading to degeneration of retinal photoreceptor cells.

Images & Validation

Tested ApplicationsIHC-P
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml
ReactivityHuman
Application Notes
Optimal dilution of the MerTK antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG2a, kappa
Clone No.MERTK/3015
ImmunogenA recombinant human partial protein (amino acids 55-148) was used as the immunogen for the MerTK antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DisclaimerFor research use only

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MerTK Antibody

IHC staining of FFPE human colon with MerTK antibody (clone MERTK/3015). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

MerTK Antibody

IHC staining of FFPE human colon carcinoma with MerTK antibody (clone MERTK/3015). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

MerTK Antibody

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using MerTK antibody (clone MERTK/3015). These results demonstrate the foremost specificity of the MERTK/3015 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

MerTK Antibody

SDS-PAGE analysis of purified, BSA-free MerTK antibody (clone MERTK/3015) as confirmation of integrity and purity.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol

MerTK Antibody (orb639575)

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Available Sizes

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20 μg
$ 370.00
100 μg
$ 750.00
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