Mesothelin Rabbit Polyclonal Antibody

SKU: orb11082

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Description

Research Area

Cancer Research, Epi / Endothelial, Tumor Biology, Varscular System

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, WB
Dilution Range	WB=1:500-2000, ELISA=1:5000-10000
Reactivity	Human
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Mesothelin (101-200/630aa)
Target	MSLN
Molecular Weight	64 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MSLN; MES; CAK 1; CAK1; CAK1 antigen; Megakaryocyte potentiating factor; Mesothelin isoform 1 precursor; MPF; Pre pro megakaryocyte potentiating factor; SMR; SMRP; Soluble MPF mesothelin related protein; MSLN_HUMAN.

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Blank control: A549 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µl in 100 µl 1X PBS containing 0.5% BSA (green).

Blank control: Hela (blue). Primary Antibody: Rabbit Anti-Mesothelin/AF647 Conjugated antibody, Dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions. Protocol, The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (0.2 µg/1x10^6 cells) for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Mcf-7 Cell Lysate at 40 ug, Primary: Anti-Mesothelin (orb11082) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 64 kD, Observed band size: 62 kD.

Quick Database Links

Gene Symbol

MSLN

UniProt

Q13421

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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