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MET Rabbit Polyclonal Antibody
SKU: orb1294310
Description
Research Area
Cancer Biology
Images & Validation
−Item 1 of 7
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500–1:1000, Immunofluorescence 1:100–1:200, Immunohistochemistry (Paraffin) 1:100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
| Target | MET / C-MET / HGFR |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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All lanes: Anti c-MET antibody at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4°C.
All lanes: Anti c-MET antibody at 1/700 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4°C.
All lanes: Anti c-MET antibody at 1/700 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4°C.
All lanes: Anti c-MET antibody at 1/800 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4°C.
All lanes: Anti c-MET antibody at 1/800 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294310 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling c-MET with orb1294310 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling c-MET with orb1294310 at 1/100.
Quick Database Links
Gene Symbol
MET / C-MET / HGFR
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
MET Rabbit Polyclonal Antibody (orb1294310)
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