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MET/HGFR Antibody

SKU: orb1440712

Description

Mouse monoclonal antibody to MET/HGFR.

Research Area

Cancer Biology, Signal Transduction

Images & Validation

Tested ApplicationsIF, IHC-P, WB
Dilution rangeIF: 1:200, WB: 1:500~1000, IHC-P: 1:10~50
ReactivityHuman, Mouse

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.6AT1785
TargetMET
Molecular Weight155541
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/AppearancePurified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
DisclaimerFor research use only

Alternative Names

Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met, MET

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MET/HGFR Antibody

Detection of endogenous Met in HepG2 cell line. 10 μg/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Abgent's different anti-Met monoclonal antibodies. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab.

MET/HGFR Antibody

Fluorescent confocal image of HepG2 cells stained with MET/HGFR Antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MET/HGFR primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

MET/HGFR Antibody

MET/HGFR Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MET/HGFR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol

MET/HGFR Antibody (orb1440712)

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Available Sizes

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80 μl
$ 290.00
DispatchUsually dispatched within 5-10 working days
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