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MET/HGFR Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution range | IF: 1:200, WB: 1:500~1000, IHC-P: 1:10~50 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 6AT1785 |
| Target | MET |
| Molecular Weight | 155541 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
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Detection of endogenous Met in HepG2 cell line. 10 μg/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Abgent's different anti-Met monoclonal antibodies. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab.

Fluorescent confocal image of HepG2 cells stained with MET/HGFR Antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MET/HGFR primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

MET/HGFR Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MET/HGFR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_000236.2, NP_001120972.1 |
|---|
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Protocol Information
MET/HGFR Antibody (orb1440712)
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