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MET/HGFR Antibody
SKU: orb1939458
Description
Research Area
Cancer Biology, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution range | IF - 1:100, IHC-P - 1:100-500 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Molecular Weight | 155541 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Disclaimer | For research use only |
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All lanes: Anti-MET/HGFR Antibody at dilution. Lane 1: HeLa whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: COS-7 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 156 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Fluorescent confocal image of HepG2 cells stained with MET/HGFR Antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MET/HGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MET/HGFR Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunohistochemical analysis of paraffin-embedded H.liver section using MET/HGFR Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
MET/HGFR Antibody (orb1939458)
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