Methotrexate

SKU: orb1308506

Description

Methotrexate is a folate analog and potent inhibitor of dihydrofolate reductase (DHFR), exhibiting antimetabolic and immunosuppressive properties. It is widely used in research areas including oncology, immunology, and rheumatology for in vitro cell-based assays and in vivo disease modeling.

Research Area

Cell Biology, Metabolism Research, Molecular Biology

Images & Validation

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Key Properties

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CAS Number	59-05-2
MW	454.44
Purity	99.91% (May vary between batches)
Formula	C₂₀H₂₂N₈O₅
SMILES	NC=1C2=C(N=CC(CN(C)C3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)=N2)N=C(N)N1
Target	Antifolate,ADC Cytotoxin,Dehydrogenase,Apoptosis,DNA/RNA Synthesis
Solubility	10% DMSO+40% PEG300+5% Tween 80+45% Saline:8.3 mg/mL (18.26 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:70 mg/mL (154.04 mM)

Bioactivity

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Target IC50	NAMALWA cells:33-133 nM\|5637 cells:0.016 μM\|NALM-6 cells:33-133 nM\|A549 cells:0.013 μM\|JURKAT cells:33-133 nM\|BGC-823 cells:0.11 μM\|HEK293 cells:1.27 μM\|Ramos cells:33-133 nM\|COLO 205 cells:3.27 μM\|NALM-16 cells:33-133 nM\|CEM cells:33-133 nM\|DAN-G cells:0.077 μM\|A427 cells:5.52 μM\|DHFR (human):24 nM\|HBL-100 cells:0.04 μM Read more...
In Vivo	METHODS: To test for chronic toxicity, Methotrexate (0.25-6 mg/kg) was administered intraperitoneally to C57BL/6, DBA/2, and C3H mice five times per week for 12-18 months. RESULTS: The 0.25-2 mg/kg dose was well tolerated with minimal cytostasis in lymphoid tissues, testis and skin. 3-6 mg/kg dose produced well-known acute to subacute hematopoietic and gastrointestinal injuries leading to early death. METHODS: To examine the mode of action in different types of rheumatoid arthritis (RA) and multiple sclerosis (MS) models, Methotrexate (0.1-5 mg/kg) was injected intraperitoneally once a day for fourteen days into RA and MS models with different pathogenesis. RESULTS: Methotrexate showed strong ameliorative effects in classical RA models such as CIA and PIA as well as in MS and EAE models.Methotrexate acts only prior to and dependent on T-cell activation in T-cell activated diseases. Read more...
In Vitro	METHODS: Six pediatric leukemia and lymphoma cell lines, NALM-6, NALM-16, JURKAT, CEM, RAMOS, and NAMALWA, were treated with Methotrexate (0.002-5 μM) for 120 h, and the proliferation inhibitory activity of the cells was assayed using the SRB method. RESULTS: The median IC50 of Methotrexate against tumor cells was 78 nM, and the IC50 of the six tumor cells ranged from 33-133 nM. METHODS: Human ovarian cancer cells SKOV-3 were treated with Methotrexate (15-50 μM) for 24 h. Apoptosis was detected using AO/EtBr probe. RESULTS: Methotrexate induced apoptosis in SKOV-3 cells. Read more...
Cell Research	Methotrexate (MTX) is dissolved in DMSO and stored, and then diluted with appropriate media before use. Each cell line is studied in growth inhibition experiments using 96-well microtiter plates. As antifols are schedule dependent, preliminary experiments are aimed at defining the longest duration of exposure that would allow for continuous logarithmic phase growth of cells without changing of the culture media while maintaining a linear relationship between SRB optical density and cell number. Twenty-four hours after cell plating, the cell lines are exposed to the antifol for 120 h (three replicates per experiment). To ensure that a complete sigmoidal survival-concentration curve could be observed, the following drug concentrations are studied: Methotrexate (0.002-5 μM), AMT (0.0001-1 μM), PXD (0.0003-10 μM), TLX (0.0002-0.5 μM). Experiments are repeated at least twice. Read more...

Storage & Handling

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Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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arthritis, antineoplastic, Apoptosis, Antifolate, Amethopterin, ADC Payload, ADC Cytotoxin, ADCCytotoxin, DNA, DNASynthesis, DNA/RNA Synthesis, DNA Synthesis, CL 14377, CL14377, CL-14377, immunosuppressant, inhibit, lymphoblastic, Methotrexate, NCI-C 04671, NCI-C04671, NCI-C-04671, Inhibitor, hDHFR(dihydrofolate reductase), leukemia, WR19039, WR-19039, WR 19039, reductase, RNA Synthesis, RNASynthesis, rheumatoid, synthesis

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID

126941 ↗

Key Properties

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No computed properties available.

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Methotrexate (orb1308506)

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Reactivity:	Human
Dynamic Range:	15.63-1000 ng/mL
Sensitivity:	4.57 ng/mL

Applications:	ELISA
Reactivity:	Chemical
Species/Host:	Sheep
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA
Reactivity:	All
Species/Host:	Camelus
Clonality:	Monoclonal
Conjugation:	Unconjugated

Applications:	ELISA
Reactivity:	All
Species/Host:	Camelus
Clonality:	Monoclonal
Conjugation:	Unconjugated

Applications:	ELISA
Reactivity:	All
Species/Host:	Mouse
Clonality:	Monoclonal
Conjugation:	Unconjugated

Methotrexate

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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