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MGMT Antibody
SKU: orb1927312
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, IHC-P, WB |
|---|---|
| Dilution range | IHC-P - 1:100-500, IF - 1:25, WB - 1:1000, IHC - 1:100 |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Molecular Weight | 21646 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Disclaimer | For research use only |
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MGMT Antibody western blot analysis in MCF-7 cell line lysates (35μg/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).
Immunohistochemical analysis of paraffin-embedded Human Prostate cancer section using Pink1. Diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. small intestine section using MGMT Antibody. Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Fluorescent image of Hela cells stained with MGMT Antibody. Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
MGMT Antibody (orb1927312)
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