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H2AFY Antibody
SKU: orb1256637
Featured
Description
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 123-372 of human H2AFY (NP_613258.2). |
| Target | H2AFY |
| Molecular Weight | Observed: 40kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H2AFY, H2A.y, H2A/y, H2AF12M, H2AFJ, MACROH2A1.1, mH2A1, macroH2A1.2
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Western blot analysis of extracts of various cell lines, using H2AFY antibody (orb1256637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney using mH2A1 antibody (orb1256637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using mH2A1 antibody (orb1256637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using mH2A1 antibody (orb1256637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung cancer using mH2A1 antibody (orb1256637) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using mH2A1 antibody (orb1256637). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
H2AFY
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
H2AFY Antibody (orb1256637)
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