CCL15 Antibody (Biotin)

SKU: orb1671449

Description

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, WB
Reactivity	Human
Application Notes	Sandwich ELISA: To detect hMIP-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human MIP-5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMIP-5. Western Blot To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Immunogen	Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hMIP-5. Anti-Human MIP-5 specific antibody was purified by affinity chromatography and then biotinylated.
Target	CCL15
Purification	MIP-5 specific antibody was purified by affinity chromatography and then biotinylated
Conjugation	Biotin

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Concentration	batch dependent
Disclaimer	For research use only

Alternative Names

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Macrophage Inflammatory Protein-5, CCL15, HCC-2, Lkn-1

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To detect hMIP-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MIP-5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMIP-5.

To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

CCL15

UniProt

Q16663

RefSeq:Q16663

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	Q16663

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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