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Mitofusin 2 Rabbit Polyclonal Antibody
SKU: orb101666
Featured
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, ICC, WB |
|---|---|
| Dilution range | WB=1:500-2000, ELISA=1:5000-10000, ICC/IF=1:50 - 1:200 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Porcine |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Mitofusin 2 (1-120/757aa) |
| Target | MFN2 |
| Molecular Weight | 83 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−CMT2A; CMT2A2; MARF; CPRP 1; CPRP1; Fzo; HSG; hyperplasia suppressor gene; Hypertension related protein 1; MFN 2; Mfn2; MFN2_HUMAN; Mitochondrial assembly regulatory factor; Mitofusin-2; Mitofusin2; Transmembrane GTPase MFN2.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Mitofusin 2) Polyclonal Antibody, Unconjugated (orb101666) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Mitofusin 2 (orb101666) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 83 kD, Observed band size: 68 kD.
Sample: Lane 1: Mouse Muscle tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Rat Muscle tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-Mitofusin 2 (orb101666) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 83 kD, Observed band size: 77 kD.
Tissue/cell: human breast cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Mitofusin 2 Polyclonal Antibody, Unconjugated (orb101666) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Mitofusin-2 Polyclonal Antibody, Unconjugated (orb101666) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated (orb868805) used at 1:200 dilution for 40 minutes at 37°C.
Quick Database Links
Gene Symbol
MFN2
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Mitofusin 2 Rabbit Polyclonal Antibody (orb101666)
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