MK5/MAPKAPK5 Antibody

SKU: orb1248427

This product can be custom made

KO/KD
Validated

Description

MK5/MAPKAPK5 Antibody

Research Area

Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC, IP, KO/KD Validated, WB
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Porcine, Rat
Application Notes	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1µg/mL.Immunohistochemistry:In paraffin embedded Human Kidney shows brushborder staining in the PCT. Recommended concentration, 4-8µg/mL.Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of the IP were labelled with mouse anti-MK5 (SC) or with rabbit anti-ERK3 serum (CST). An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression constructas described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Immunogen	The immunogen for this antibody is: C-STEALDNVLPSAQ
Target	MK5 / MAPKAPK5
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MAPKAPK5, mitogen-activated protein kinase-activated protein kinase 5, MAPKAP-K5, MK-5, MK5, PRAK, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, p38-regulated/activated protein kinase

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Quick Database Links

Gene Symbol

MK5 / MAPKAPK5

UniProt

Q8IW41

RefSeq (Protein):NP_003659.2, NP_620777.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_003659.2, NP_620777.1

Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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Applications:	ELISA, IHC, IP, WB
Predicted Reactivity:	Bovine, Canine, Porcine, Rat
Reactivity:	Human, Mouse
Species/Host:	Goat
Clonality:	Polyclonal
Conjugation:	Unconjugated

MK5/MAPKAPK5 Antibody

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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