ML241 hydrochloride

SKU: orb1302655

Description

ML241 is a potent and selective p97 ATPase inhibitor, demonstrated to block the degradation of p97-dependent proteasome substrates in cellular assays. This activity provides a rationale for its investigation as a potential chemotherapeutic agent in cancer research, supporting the development of pathway-specific p97 inhibitors for both in vitro and in vivo studies.

Research Area

Protein Biochemistry

Images & Validation

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Key Properties

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CAS Number	2070015-13-1
MW	408.92
Purity	99.96% (May vary between batches)
Formula	C₂₃H₂₅ClN₄O
SMILES	Cl.C(Nc1nc(nc2CCCCc12)N1CCOc2ccccc12)c1ccccc1
Target	p97
Solubility	DMSO:50 mg/mL (122.27 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.89 mM)

Bioactivity

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Target IC50	p97:100 nM
In Vitro	ML241 is cytotoxic to HCT15 and SW403 cells, with GI50s of 53 and 33 μM after treatment for 24 h, and 13 and 12 μM after treatment for 72 h, respectively Read more...
Cell Research	HeLa cells stably expressing ODD-luciferase are seeded onto a 96-well white solid bottom plate (5000 cells/well) and cells are grown for 16 h. Cells are treated with DMEM containing MG132 (4 μM) for 1h and washed with 100 μL PBS twice. DMEM containing 2.5% FBS, cycloheximide (50 μg/mL) and ML241 are added into the well. Four 96-well plates are prepared and one of the plates is taken out from incubator at each time point (70, 90, 120, or 150 min). Luciferin (50 μL of 1 mg/mL in PBS) is added into each well containing 50 μL of medium and incubated at room temperature with shaking at 500 rpm for 5 min. Luminescence intensity is determined with 0.1 ms integration time on the Synergy HT Microplate Reader Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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inhibit, Inhibitor, p97, ML 241, ML 241 Hydrochloride, ML241, ML-241, ML241 hydrochloride, ML241 Hydrochloride, ML-241 hydrochloride, ML-241 Hydrochloride, Cdc48, VCP

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Compound Identifiers

PubChem CID

119081413 ↗

Key Properties

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No computed properties available.

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