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Recombinant MLH1 Antibody / MutL Homolog 1
SKU: orb1151494
Description
Research Area
Cancer, DNA Damage, Epigenetics
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P |
|---|---|
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | Rabbit IgG |
| Clone No. | MLH1/6284R |
| Immunogen | Recombinant full-length human MLH1 protein was used as the immunogen for the recombinant MLH1 antibody. |
| Purification | Protein A/G affinity |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Recombinant MLH1 Antibody / MutL Homolog 1 [orb2636618]
IHC-P
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Rabbit
Recombinant
Unconjugated
100 μg
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IHC staining of FFPE human colon carcinoma tissue with recombinant MLH1 antibody (clone MLH1/6284R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE Lynch Syndrome / Hereditary Non-Polyposis Colorectal Cancer (HNPCC) with recombinant MLH1 antibody (clone MLH1/6284R) at 2 ug/ml in PBS, 30 min RT. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human Hereditary Nonpolyposis Colorectal Cancer (HNPCC) with recombinant MLH1 antibody (MLH1/6284R) at 2 ug/ml.
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Recombinant MLH1 Antibody / MutL Homolog 1 (orb1151494)
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