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MMP14 Rabbit Polyclonal Antibody
SKU: orb1294372
Description
Images & Validation
−Item 1 of 7
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500, Immunofluorescence 1:150-1:300, Immunohistochemistry (Paraffin) 1:100-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Human MMP14 / MT1-MMP |
| Target | MMP14 / MT1-MMP |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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All lanes: Anti-MMP14 / MT1-MMP antibody - Loading Control at 1/500 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with orb1294372 overnight at 4°C.
All lanes: Anti-MMP14 / MT1-MMP antibody - Loading Control at 1/500 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with orb1294372 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling MMP14 / MT1-MMP with orb1294372 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling MMP14 / MT1-MMP with orb1294372 at 1/100.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294372 anti-MMP14 / MT1-MMP antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294372 anti-MMP14 / MT1-MMP antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling MMP14 / MT1-MMP with orb1294372 at 1/100.
Quick Database Links
Gene Symbol
MMP14 / MT1-MMP
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
MMP14 Rabbit Polyclonal Antibody (orb1294372)
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