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Description
Images & Validation
−| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution Range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, IHC-P: 1:10~50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This MMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human MMP3. Antigen Region: 30-59 aa. |
| Target | MMP3 |
| Molecular Weight | 53977 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−MMP3 Antibody (N-term) [orb1936610]
IF, IHC-P, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl

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Confocal immunofluorescent analysis of MMP3 Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of lysates from Raji, HepG2, NCI-H460, Ramos cell line (from left to right), using MMP3 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

MMP3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MMP3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

MMP3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MMP3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_002413.1 |
|---|
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Protocol Information
MMP3 Antibody (N-term) (orb1788400)
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