Mouse ADP Ribosyltransferase 1 (ART1) ELISA Kit

SKU: orb780613

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADP Ribosyltransferase 1(ART1).

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ART1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ART1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ART1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ART1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.79-50 ng/mL
Sensitivity	0.31 ng/mL
Concentration	50 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD296 ELISA Kit, ART2 ELISA Kit, ARTC1 ELISA Kit, Mono(ADP-ribosyl)transferase 1 ELISA Kit, ADP-ribosyltransferase C2 and C3 toxin-like 1 ELISA Kit, GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1 ELISA Kit

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Mouse ADP Ribosyltransferase 1 (ART1) ELISA Kit

Quick Database Links

UniProt

P52961

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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