Mouse Alanine Aminotransferase (ALT) ELISA Kit

SKU: orb775785

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alanine Aminotransferase(ALT).

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Tested Applications

ELISA

Application Notes

standard: 100 U/L. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ALT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ALT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ALT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ALT in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 U/L
Sensitivity	0.64 U/L
Concentration	100 U/L

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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GPT ELISA Kit, ALAT ELISA Kit, SGPT ELISA Kit, AAT1 ELISA Kit, GPT1 ELISA Kit, Serum Glutamic Pyruvic Transaminase ELISA Kit, Alanine Transaminase ELISA Kit, Glutamate pyruvate transaminase 1 ELISA Kit, Glutamic--alanine transaminase 1 ELISA Kit

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Mouse Alanine Aminotransferase (ALT) ELISA Kit

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UniProt

Q8QZR5

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ELISA (2)

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Mouse (2)

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PubMed: 39388218

Applications

ELISA

Reactivity

Mouse

Agata Tomaszewska 1 2, Bartłomiej Kost 3, Marek Brzeziński 3, Martyna Nowicka 4 5 6, Maja Krupa 4, Tomasz Rechciński 7, Łucja Balcerzak 8, Sława Glińska 8, Magdalena Chmiela 4, Agnieszka Krupa Targeted and safe delivery of colchicine via polymeric nanocarriers for potential atherosclerosis therapy with in vitro and in vivo evaluation Sci Rep, (2025)

PubMed: 40858667

Applications

ELISA

Reactivity

Mouse